Cell type specific mechanoadaptation to sustained hydrostatic pressure and flow in glioblastoma cells and astrocytes

This dataset includes results presented in the paper entitled:"Cell type specific mechanoadaptation to sustained hydrostatic pressure and flow in glioblastoma cells and astrocytes" List of data in this dataset: WST-1 Trypan Blue GBMData on U87-MG cell viability assessed by WST-1 assay and cell counts determined using Trypan Blue exclusion under experimental conditions. p53 Ki67 GBMQuantification of p53- and Ki-67-positive U87-MG cells under experimental conditions. Elongation Index GBMData used to calculate the elongation index (ratio of major to minor axes) in U87-MG cells under experimental conditions. EGFR ExpressionData on EGFR-positive surface area per U87-MG cell under experimental conditions. ROS analysis GBMPercentage of ROS-positive and ROS-negative U87-MG cells under experimental conditions. qPCR analysis GBMGene expression analysis in U87-MG cells under experimental conditions. Supplementary Data S1 Matrice_significative_HP_vs_C_U87MG_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of hydrostatic pressure (HP) vs control in U87-MG cells. Supplementary Data S2 Matrice_significative_F_vs_C_U87MG_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of flow (F) vs control in U87-MG cells. Supplementary Data S5 Matrice_significative_HP_vs_F_U87MG_pvalue_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of hydrostatic pressure (HP) vs flow (F) in U87-MG cells. enrichment_HP vs C_Up in HP_U87Excel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in HP vs control in U87-MG cells. enrichment_HP vs C_Up in C_U87Excel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in control vs HP in U87-MG cells. enrichment_HP vs F_Up in HP_pvalue_U87Excel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in HP vs flow (F) in U87-MG cells (p-value based). enrichment_HP vs F_Up in F_pvalue_U87Excel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in flow (F) vs HP in U87-MG cells (p-value based). Matrice_tot_Ttest_U87Excel file (.xlsx) containing the complete quantitative proteomics dataset for U87-MG cells, including protein abundances, Gene Ontology annotations, and Student’s t-test results (p-values, q-values, and fold differences) for all comparisons. WST-1 Trypan Blue AstrocytesData on astrocyte viability assessed by WST-1 assay and cell counts determined using Trypan Blue exclusion under experimental conditions. p53 Ki67 AstrocytesQuantification of p53- and Ki-67-positive astrocytes under experimental conditions. Elongation Index AstrocytesData used to calculate the elongation index (ratio of major to minor axes) in astrocytes under experimental conditions. ROS analysis AstrocytesPercentage of ROS-positive and ROS-negative astrocytes under experimental conditions. qPCR analysis AstrocytesGene expression analysis in astrocytes under experimental conditions. Supplementary Data S4 Matrice_significative_HP_vs_C_Astrociti_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of hydrostatic pressure (HP) vs control in astrocytes. Supplementary Data S5 Matrice_significative_F_vs_C_Astrociti_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of flow (F) vs control in astrocytes. Supplementary Data S6 Matrice_significative_HP_vs_F_Astrociti_pvalue_ClusterExcel file (.xlsx) containing quantitative proteomics data and statistical analysis results for the comparison of hydrostatic pressure (HP) vs flow (F) in astrocytes. enrichment_HP vs C_Up in HP_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in HP vs control in astrocytes. enrichment_HP vs C_Up in C_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in control vs HP in astrocytes. enrichment_F vs C_Up in F_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in flow vs control in astrocytes. enrichment_F vs C_Up in C_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in control vs flow in astrocytes. enrichment_HP vs F_Up in HP_pvalue_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in HP vs flow (F) in astrocytes (p-value based). enrichment_HP vs F_Up in F_pvalue_AstroExcel file (.xlsx) with Gene Ontology (GO) enrichment results for proteins upregulated in flow (F) vs HP in astrocytes (p-value based). Matrice_tot_Ttest_AstrocytesExcel file (.xlsx) containing the complete quantitative proteomics dataset for astrocytes, including protein abundances, Gene Ontology annotations, and Student’s t-test results (p-values, q-values, and fold differences) for all comparisons.