Next Generation Sequencing Facilitates Quantitative Analysis of keloid and normal skin

Purpose: This study aimed to investigate the role of dysregulated circRNAs in keloid. Methods: High-throughput sequencing was used to detect the changes of circRNAs, microRNAs(miRNAs) and mRNAs in 3 samples of keloid and corresponding normal skin.Based on Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway enrichment analysis combining several signaling pathways associated with keloid formation and progression, we screened these differentially expressed circRNAs.qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: Compared with normal controls, there were an average of 120 and 12 circRNAs, 44 and 63 miRNAs, 656 and 156 mRNAs were up-regulated and down-regulated, respectively, in keloids. Conclusions: This study implicates that the abnormal expression of circRNAs in cases of keloid may correlate with its pathological process. Overall design: Profiling of Differentially Expressed Circular RNAs in keloid tissue.