Transcriptome sequencing of NPV treated cells and resuscitated cells derived from MEFs after TSZ-induced necroptosis.

To investigate why resuscitated cells secrete enhanced levels of pro-repair factors, we established three experimental groups. The control group consisted of untreated mouse embryonic fibroblast (MEFs). The C+NPVs group was prepared by incubating normal MEFs with NPVs. For the Res group, MEFs were treated with TSZ, then digested and sorted by FACS to obtain agonal cells. These agonal cells were subsequently incubated with NPVs. After medium replacement to remove non-adherent, terminally dead cells, the remaining adherent resuscitated cells were collected as the Res group.