Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism. However, it is difficult to identify the mutant gene(s) due to cells are usually mutagen

NIAID Data Ecosystem30

Arabidopsis thaliana Raw sequence reads. Arabidopsis thaliana

DNA损伤修复

正向遗传筛选

Forward-directed genetic screen aiming at the identification of the genes involved in the repair of zebularine-induced DNA damage. The candidates selected for mapping were back-crossed to the non-muta

NIAID Data Ecosystem10

Table_2_TDNAscan: A Software to Identify Complete and Truncated T-DNA Insertions.xlsx

T-DNA插入检测

正向遗传筛选

Transfer (T)-DNA insertions in mutants isolated from forward genetic screens are typically identified through thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), inverse PCR, or plasmi

NIAID Data Ecosystem30

Caenorhabditis elegans Genome sequencing. Caenorhabditis elegans

基因组测序

正向遗传筛选

Forward screening.

NIAID Data Ecosystem10