Small-Molecule-Induced ERBB4 Activation to Treat Heart Failure

To determine the transcriptomic changes of small-molecule-induced activation of ERBB4 in heart failure, we tested the effects of the two small molecules (NRG1 and EF-1) of ERBB4 agonists on iAM (immortalized atrial cardiomyocytes) model. The transcriptional profiles of mRNA in these samples will be measured with high throughput technology. Changes in transcriptional profiles between the activation of EF-1 and NRG1 will be compared.为探究小分子诱导ERBB4激活引发心力衰竭过程中的转录组学变化，本研究针对两种ERBB4激动剂小分子——NRG1与EF-1，在永生化心房心肌细胞（immortalized atrial cardiomyocytes，简称iAM）模型中验证其干预效应。后续将采用高通量技术检测上述样本的mRNA转录组谱，并对比EF-1与NRG1分别激活ERBB4后的转录组谱差异。