Whole genome sequencing of 415 haploid strains derived from natural diploid isolates of Saccharomyces cerevisiae

A collection of 415 haploid MATalpha ura3delta0 ho::ZeocinR-BC strains derived from 771 diverse natural diploid isolates was generated and sequenced using illumina single and/or paired-end sequencing technique. Strains are tagged with a unique 20 bp molecular barcode at the HO locus, for which the native HO gene has been replaced with a Zeocin resistance marker and the barcode using CRISPR-Cas9 based editing.