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Frequency and potency of binders derived from synthetic diversity.

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https://figshare.com/articles/dataset/_Frequency_and_potency_of_binders_derived_from_synthetic_diversity_/257459
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Information is provided on the SnA and SiB sequences found by screening positive clones in scFv ELISA after three rounds of selection against hIFNγ. Corresponding VH frequencies by NGS are described. Full circles and white triangles highlight identical CDRH3 in both SnA and SiB groups. Clones were purified as scFv and tested in dose response ELISA against hIFNγ, the last column shows the EC50 values observed in nM (n = 2).
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2012-08-24
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