H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin

H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin e13.5 supraorbital cranial mesenchyme was manually dissected. The sample collected consisted of all the tissue above the brain which included the cranial mesenchyme plus the ectoderm. Chromatin immunoprecipitation and sequencing (ChIP-seq) was then performed by Active Motif. 14 µg chromatin was immunoprecipitated with 4 µg rabbit anti-H3K27me3 (Millipore #07-449). Sequencing was performed on an Illumina NextSeq 500 producing 75-nucleotide, single end reads. Drosophila DNA was “spiked in” and used to normalize the number of reads in the samples. Reads were aligned using Bowtie2 to the mouse mm9 genome and peaks were called using Macs 1.4 at default settings. contributor: Case Western Reserve University