Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs. Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs

A variety of base editors have been developed to achieve C-to-T editing in different genomic contexts. Here, we compare a panel of five base editors on their C-to-T editing efficiencies and product purity at commonly-editable sites, including some human pathogenic C-to-T mutations. We further profile the accessibilities of twenty base editors to all possible pathogenic mutations in silico. Finally, we build the BEable-GPS (Base Editable prediction of Global Pathogenic SNVs) database for users to select proper base editors to model or correct disease-related mutations. This in-vivo comparison and in-silico profiling catalogs the availability of base editors and their broad applications in biomedical studies. Overall design: Comparison of cytosine base editors on pathogenic and genomic sites