Transcriptome of the visual cortex in WT or Chrdl1 KO mice at post-natal day 14

Chrdl1, a BMP antagonist, is an astrocyte-secreted factor that promotes synapse maturation in the upper layers of the visual cortex at P14. We analyzed the transcriptome of the visual cortex of WT and Chrdl1 KO mice at P14 in order to determine if genes related to BMP signaling, astrocyte development or synapse formation were altered. RNA was isolated from visual cortex of WT or KO mice at post-natal day 14.Chrdl1是一种骨形态发生蛋白（Bone Morphogenetic Protein, BMP）拮抗剂，同时也是星形胶质细胞分泌的因子，可在出生后第14天（post-natal day 14, P14）促进视觉皮层上层的突触成熟。为探究与BMP信号通路、星形胶质细胞发育或突触形成相关的基因是否存在差异表达，本研究对该日龄的野生型（Wild Type, WT）及Chrdl1基因敲除（Knockout, KO）小鼠的视觉皮层转录组开展了分析。实验中，我们从该日龄的野生型或基因敲除小鼠的视觉皮层中分离得到RNA。