Relative contributions of various endogenous and exogenous factors to the mosquito microbiota

Researchers used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. This data was used to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition.

研究人员采用基于高通量测序的检测方法，对665只野外捕获的个体蚊子的细菌组成和多样性进行了表征，同时分析了它们的物种、抗药基因位点的基因型、血液成分以及携带的真核寄生虫和病毒。该数据被用于严格评估每个参数对细菌多样性的个体影响，以及每个参数对微生物组成的相对贡献。