GNPS - Quantification N-acyl lipids (stool samples and microbial monocultures)

Stool samples are part of the MSV000092833 dataset (HNRC cohort). Microbial monocultures contained histamine, cadaverine, and putrescine added to the media. MS/MS data was acquired on a Q Exactive Orbitrap in positive ionization mode. Quantification of N-acyl lipids was performed (histamine-C2:0, histamine-C3:0, histamine-C4:0, histamine-C5:0, cadaverine-C2:0, cadaverine-C3:0, cadaverine-C5:0, cadaverine-C6:0, and dopamine-C2:0), while retention time matching was also done for histamine-C6:0, cadaverine-C7:0, serotonin-C2:0, and tryptophan-C3:0. All the N-acyl lipids used were acquired as pure standards from commercial vendors.