Data underlying the publication: Interfacial protein adsorption behavior can be connected across a wide range of timescales using the microfluidic EDGE (Edge-based droplet GEneration) tensiometer

# IntroductionIn the current study, we expand the use of our EDGE (Edge-based Droplet GEneration) device, and validate the findings with ADT (automated drop tensiometer) results at complementary timescales.Furthermore, we explore if the EDGE tensiometer could distinguish effects occurring at high whey protein concentrations (2.5 – 10 wt%).In this dataset, you can find an excel file with an overview of all dataset named "EDGEvsADT_WPI_20240516_v09_final.xlxs" (in the Analyzed_Data folder).You can also find the raw data in the Raw_Data folder with .txt files.Finally, in the Plotted_Data_Scripts folder you can find all processed data from the excel file in .txt extension together with a Matlab code to plot the data.<br># AbstractHypothesisOur hypothesis is that dynamic interfacial tension values as measured by the partitioned-Edge-based Droplet GEneration (EDGE) tensiometry can be connected to those obtained with classical techniques, such as the automated drop tensiometer (ADT), expanding the range of timescales towards very short ones.<br>ExperimentsOil-water and air-water interfaces are studied, with whey protein isolate solutions (WPI, 2.5 – 10 wt%) as the continuous phase.The dispersed phase consists of pure n-hexadecane or air. The EDGE tensiometer and ADT are used to measure the interfacial (surface) tension at various timescales.A comparative assessment is carried out to identify differences between protein concentrations as well as between oil-water and air-water interfaces.<br>FindingsThe EDGE tensiometer can measure at timescales down to a few milliseconds and up to around 10 seconds, while the ADT provides dynamic interfacial tension values after at least one second from droplet injection and typically is used to also cover hours.The interfacial tension values measured with both techniques exhibit overlap, implying that the techniques provide consistent and complementary information.Unlike the ADT, the EDGE tensiometer distinguishes differences in protein adsorption dynamics at protein concentrations as high as 10 wt% (which is the highest concentration tested) at both oil-water and air-water interfaces.<br># Measurements and data collectionMeasurements and data collection were done according to what is described in the paperWe used two main setups and equipment, namely ADT (Automated Drop Tensiometer) and EDGE (partitioned-Edge-based Droplet GEneration) for data collection

# 引言
在本研究中，我们拓展了EDGE（Edge-based Droplet GEneration，基于边缘液滴生成）装置的应用，并通过互补时间尺度下的ADT（automated drop tensiometer，全自动滴形张力仪）实验结果对研究发现进行验证。此外，我们探究了EDGE张力仪能否区分高乳清蛋白浓度（2.5~10 wt%）下的相关效应。
本数据集的Analyzed_Data文件夹中包含一份汇总所有数据集的Excel文件，文件名为EDGEvsADT_WPI_20240516_v09_final.xlsx。Raw_Data文件夹中则存储了原始的.txt格式数据文件。最后，Plotted_Data_Scripts文件夹中包含了从上述Excel文件导出的所有经过处理的.txt格式数据，以及用于绘制数据图表的Matlab代码。

# 摘要
## 研究假设
我们的假设为：基于分区式EDGE（partitioned-Edge-based Droplet GEneration，边缘液滴生成）张力法测得的动态界面张力值，可与经典技术（如全自动滴形张力仪ADT）所得结果相互关联，从而将可观测的时间尺度拓展至极短区间。

## 实验设计
本研究以乳清分离蛋白（whey protein isolate, WPI）溶液（浓度范围2.5~10 wt%）作为连续相，探究油-水界面与气-水界面的相关特性；分散相为正十六烷纯品或空气。我们采用EDGE张力仪与ADT两种设备，在不同时间尺度下测量界面（表面）张力，并通过对比分析，识别不同乳清蛋白浓度下以及油-水、气-水界面间的差异。

## 研究结果
EDGE张力仪可实现数毫秒至约10秒区间内的测量；而ADT则在液滴注入至少1秒后开始采集动态界面张力数据，其常规观测范围可覆盖至数小时。两种设备测得的界面张力值存在重叠区间，表明二者可提供一致且互补的实验数据。与ADT不同的是，EDGE张力仪能够在油-水与气-水两种界面下，区分最高至10 wt%（本研究测试的最高浓度）的乳清蛋白吸附动力学差异。

# 测量与数据采集
本研究的测量与数据采集流程严格遵循论文中的描述，主要采用ADT（Automated Drop Tensiometer，全自动滴形张力仪）与EDGE（partitioned-Edge-based Droplet GEneration，分区式边缘液滴生成装置）两套设备完成数据采集。