Transcriptional profiling of striatum from the Q140 mouse model of Huntington's disease, from A rare genetic variant confers resistance to neurodegeneration across multiple neurological disorders by augmenting selective autophagy

Striatum isolated from 6 m/o wildtype, Q140 knock-in mice in the presence or absence of Alfy overexpression. Alfy overexpression achieved by knockin of human WDFY3 cDNA into Rosa26 locus. Total RNA seq analysis. Striata from 6 m/o Q140 and WT in the presence or absence of Alfy overexpression. Total RNA. 4 males and 4 females per genotype. N=8 per group. Littermates used本实验数据集的样本为从6月龄野生型（wildtype）与Q140敲入（Q140 knock-in）小鼠体内分离得到的纹状体（striatum），涵盖存在Alfy过表达（Alfy overexpression）与不存在Alfy过表达两种实验条件。Alfy过表达通过将人源WDFY3互补脱氧核糖核酸（human WDFY3 cDNA）敲入Rosa26位点（Rosa26 locus）实现。本次实验采用总RNA测序（total RNA sequencing）开展分析。实验样本均来自6月龄Q140及野生型小鼠，覆盖存在或不存在Alfy过表达的组别，提取材料为总RNA。每组包含4只雄性与4只雌性小鼠，样本量为N=8，实验所用小鼠均为同窝仔鼠（littermates）。