Genome-Wide Identification of Conditionally Essential Genes Supporting Streptococcus suis Growth in Serum and Cerebrospinal Fluid – Supplementary Dataset

This dataset contains supplementary data for the study “Genome-Wide Identification of Conditionally Essential Genes Supporting <em>Streptococcus suis</em> Growth in Serum and Cerebrospinal Fluid.” The research aimed to identify conditionally essential genes that facilitate <em>S. suis</em> survival in host environments. Using transposon sequencing (Tn-seq) combined with Oxford Nanopore sequencing, the study profiled mutant fitness in active porcine serum and cerebrospinal fluid derived from human choroid plexus organoids. The dataset includes Tn-seq output files, gene annotations, and raw and processed data supporting Figures 2 to 6 of the manuscript and Supplementary Tables S1 to S3. A detailed README file is included to describe the content and structure of each dataset component. The raw FAST5 and FASTQ sequencing files are available in the European Nucleotide Archive (ENA) under study accession number PRJEB89170. The Tn-Seq analysis pipeline developed for this study, Nanopore-TnSeq-Pipeline, is also available and can be accessed via a link provided in both the manuscript and this data repository.