Bulk RNA-sequencing Analysis of the Acute Myeloid Leukemia(AML) Malignant Bone Marrow (BM) Infiltrated Tregs

By RNA-sequencing, we compared four different bone marrow (BM)-derived Tregs, and found that the gene expression profile from No Tumor transplanted group and ST2-KO Tregs tansplanted group shown similar pattern, while WT Treg and Tbet-KO Tregs transplanted group shown the similar trend. Overall design: WT T conventional cells (Tcon) plus WT Treg transplanted group (No Tumor); WT T conventional cells (Tcon) plus WT Tregs plus MLL-AF9 leukemic cells transplanted group (WT Treg); WT T conventional cells (Tcon) plus ST2-KO Treg plus MLL-AF9 leukemic cells group (ST2-KO Treg); WT T conventional cells (Tcon) plus Tbet-KO Treg plus MLL-AF9 leukemic cells group (Tbet-KO Treg);