Isolation, identification and genomic analysis of a bacteriophage from avian Escherichia coli

a bacteriophage was isolated and purified from duck feces collected from chicken and duck shops in vegetable markets by using double-layer plate method with standard e. coli ATCC 25922 as the host strain. The plaque size was about 1mm, and the observation under the perspective electron microscope showed that. After testing, the titer of the offspring phage produced when the multiplicity of infection was 0.01 was the highest. The results of one-step growth curve showed that the titer of the phage increased sharply 30 min before and reached the stable stage after about 50 min. The temperature tolerance range of the bacteriophage is 37 to 50; The acid-base tolerance range was pH 3 to 11.