miRNAlipidomics.zip

We present here lipidomic data from HepG2 cell lysates treated with miRNA mimics (NT, miR-16, miR-30b, miR-30c). NT stands for non-targeting control siRNA, and miRNA mimics are ready-to-transfect mimics that behave like endogenous miRNAs. Lipid extraction was performed according to the method Bligh and Dyer. The following lipid species were added as internal standards: PC 14:0/14:0, PC 22:0/22:0, PE 14:0/14:0, PE 20:0/20:0 (di-phytanoyl), PS 14:0/14:0, PS 20:0/20:0 (di-phytanoyl), PI 17:0/17:0, LPC 13:0, LPC 19:0, LPE 13:0, Cer d18:1/14:0, Cer 17:0, D7-FC, CE 17:0, and CE 22:0. The residues were dissolved either in 10 mM ammonium acetate in methanol/chloroform (3:1, v/v) (for low mass resolution tandem mass spectrometry), or chloroform/methanol/2-propanol (1:2:4 v/v/v) with 7.5 mM ammonium formate (for high resolution mass spectrometry). The analysis of lipids was performed by direct flow injection analysis (FIA) using either a triple quadrupole mass spectrometer (FIA-MS/MS; QQQ triple quadrupole) or a hybrid quadrupole-Orbitrap mass spectrometer. Data processing was performed using the ALEX software which includes peak assignment and intensity picking. The extracted data were exported to Microsoft Excel 2010 and further processed by self-programmed Macros.