Table_1_m6A Methylation Analysis of Differentially Expressed Genes in Skin Tissues of Coarse and Fine Type Liaoning Cashmere Goats.docx

N6-methyladenosine (m6A) is the most common internal modification in mRNAs of all higher eukaryotes. Here we perform two high-throughput sequencing methods, m6A-modified RNA immunoprecipitation sequence (MeRIP-seq) and RNA sequence (RNA-seq) to identify key genes with m6A modification in cashmere fiber growth. A total of 9,085 m6A sites were differentially RNA m6A methylated as reported from by MeRIP-seq, including 7,170 upregulated and 1,915 downregulated. In addition, by comparing m6A-modified genes between the fine-type Liaoning cashmere goat (FT-LCG) and coarse-type Liaoning Cashmere Goat (CT-LCG) skin samples, we obtain 1,170 differentially expressed genes. In order to identify the differently methylated genes related to cashmere fiber growth, 19 genes were selected to validate by performing qRT-PCR in FT-LCG and CT-LCG. In addition, GO enrichment analysis shows that differently methylated genes are mainly involved in keratin filament and intermediate filament. These findings provide a theoretical basis for future research on the function of m6A modification during the growth of cashmere fiber.

N6-甲基腺苷（m6A）是所有高等真核生物mRNA中最常见的内部修饰。本研究采用两种高通量测序技术，即m6A修饰RNA免疫沉淀测序（MeRIP-seq）和RNA测序（RNA-seq），旨在鉴定影响 Cashmere 纤维生长的关键基因中具有m6A修饰的基因。根据MeRIP-seq报告，共鉴定出9,085个差异RNA m6A甲基化位点，其中7,170个上调，1,915个下调。此外，通过比较细毛型辽宁山羊（FT-LCG）和粗毛型辽宁山羊（CT-LCG）皮肤样本中的m6A修饰基因，我们获得了1,170个差异表达基因。为了鉴定与Cashmere纤维生长相关的差异甲基化基因，我们从FT-LCG和CT-LCG中选取了19个基因进行qRT-PCR验证。此外，GO富集分析显示，差异甲基化基因主要参与角蛋白丝和中间丝的形成。这些发现为未来研究m6A修饰在Cashmere纤维生长过程中的功能提供了理论依据。