Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient E9.5 yolksac

Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) protein. It is localized in cytosol and binds to pancreatic-type ribonucleases and inhibit their function. However the entire biological role for Rnh1 is unknown. We generated Rnh1 knock out mice by homologous recombination. Here we studied differential gene expression from wild type (Rnh1 +/+), Heterozygous (Rnh1+/-) and Knock out (Rnh1-/-) yolk sacs isolated from embryonic day 9.5 (E9.5). We used microarrays to study global gene expression regulated by Rnh1 in yolk sacs. Total RNA was isolated from E9.5 yolk sacs of Rnh1 Wild type, heterozygous and knock out.核糖核酸酶抑制剂（Ribonuclease Inhibitor，简称RI，又称Rnh1）是一种分子量为50 kDa的广泛表达的亮氨酸富集重复序列（LRR）蛋白。该蛋白定位于细胞质基质，可结合胰腺型核糖核酸酶并抑制其活性，但目前Rnh1的完整生物学功能仍未明确。本研究通过同源重组技术构建了Rnh1基因敲除小鼠，对胚胎发育第9.5天（E9.5）分离得到的野生型（Rnh1+/+）、杂合型（Rnh1+/-）及基因敲除型（Rnh1-/-）小鼠卵黄囊开展差异基因表达分析。本研究采用基因芯片（microarrays）技术，解析Rnh1在小鼠卵黄囊中调控的全基因表达谱，实验所需总RNA提取自上述E9.5时期Rnh1野生型、杂合型及基因敲除型小鼠的卵黄囊。