Effect of dexamethasone treatment alone on inflammatory mRNA expression.
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A549 cells were either not treated or stimulated with IL-1β (1 ng/ml), dexamethasone (Dex) (1 µM) or a combination of the two for 1, 2, 6 or 18 h prior to harvesting for real-time PCR analysis of 39 inflammatory genes and GAPDH. Data (n = 3) normalised to GAPDH are presented as means ± SE for genes showing greater than 2-fold induction by dexamethasone at any time point. Significance relative to non-stimulated samples was tested at time points showing greater than 2-fold induction using a paired t-test and is indicated:*, P**, P***, P
创建时间:
2015-12-02



