RNA-seq of pathogenic Escherichia coli strains CFT073 and EDL933 wild-type, rpoN mutant, and rpoN mutant + rpoN strains. RNA-seq of pathogenic Escherichia coli strains CFT073 and EDL933 wild-type, rpoN mutant, and rpoN mutant + rpoN strains

Experiment conducted to investigate the genetic factors associated with variable and heterogeneous non-planktonic in a mutant lacking rpoN unique to CFT073 bacteria. The aim was to try to understand why RpoN suppresses this heterogeneity in the wild-type during colony growth, but also asses why this phenotype does not occur in other pathogenic strains by also sequencing transcripts from EHEC EDL933. The rpoN mutant strains was complemented with plasmid-borne rpoN containing its native promoter to ensure any differences in gene expression could solely be attributed to the absence of rpoN alone.