Transcriptome of stem cell derived hepatocytes and hepG2 cells under conditions of amino acid supplementation

We report that the addition of high amounts of amino acids, combined with the overexpression of transcription factors, results in a shift of the global transcriptome of both stem cell derived hepatocytes and HepG2 cells towards the profile of primary human hepatocytes, Overall design: HepG2 cells were either grown for 5 days in standard DMEM 10% FBS medium, or in medium suppelmented with high amounts of amino acids. Stem cell derived hepatocyets were differentiated towards the hepatic lineage without (HLC) or with the overexpression of 3 (HC3X) or 6 (HC6X) transcription factors, and this in standard medium or in medium supplemented with high levels of amino acids.