CCDB_8166.jpg

Processed mosaic of a sagittal section through the brain of mouse model
of glioma. To produce transformed cells, creGFAP mice were injected in
the cortex with Ras/s.i.p53 virus and perfused after 8 weeks. In this
system, GFP is introduced by the virus and expression is induced by an
IRES site in the mRNA. Thus, the GFP gene is expressed whenever the
virus infects a cell expressing GFAP (green). The section was
immunolabeled for nestin (red) and counterstained with DAPI (blue). See
MP:8011 for a section from the same brain labeled with GFAP.

经处理的鼠模型胶质瘤大脑矢状切面镶嵌图像。为生成转化细胞，通过向皮质注射Ras/s.i.p53病毒并经8周后灌注，对creGFAP小鼠进行处理。在该系统中，病毒引入GFP蛋白，其表达由mRNA中的IRES位点诱导。因此，每当病毒感染表达GFAP（绿色）的细胞时，GFP基因即被表达。切片经巢蛋白（红色）免疫染色，并用DAPI（蓝色）进行复染。欲了解更多信息，请参阅MP:8011中标记有GFAP的同一脑部切片。