DataSheet1_Water content estimation of conifer needles using leaf-level hyperspectral data.docx

Water is a crucial component for plant growth and survival. Accurately estimating and simulating plant water content can help us promptly monitor the physiological status and stress response of vegetation. In this study, we constructed water loss curves for three types of conifers with morphologically different needles, then evaluated the applicability of 12 commonly used water indices, and finally explored leaf water content estimation from hyperspectral data for needles with various morphology. The results showed that the rate of water loss of Olgan larch is approximately 8 times higher than that of Chinese fir pine and 21 times that of Korean pine. The reflectance changes were most significant in the near infrared region (NIR, 780-1300 nm) and the short-wave infrared region (SWIR, 1300–2500 nm). The water sensitive bands for conifer needles were mainly concentrated in the SWIR region. The water indices were suitable for estimating the water content of a single type of conifer needles. The partial least squares regression (PLSR) model is effective for the water content estimation of all three morphologies of conifer needles, demonstrating that the hyperspectral PLSR model is a promising tool for estimating needles water content.