Data from: Development of HPLC-ELSD method for determination of phytochelatins and glutathione in Perilla frutescens under cadmium stress condition

A rapid, accurate and simple method was developed for the simultaneous
determination of glutathione (GSH) and phytochelatins (PCs) by high
performance liquid chromatography (HPLC) with evaporative light-scattering
detector(ELSD). GSH, Phytochelatin 2 (PC2), phytochelatin 3 (PC3),
phytochelatin 4 (PC4), phytochelatin 5 (PC5) and phytochelatin 6 (PC6) can
be separated with baseline separation within 9 minutes using a Venusil AA
column (250 mm × 4.6 mm i.d., 5 μm particle sizes). The acetonitrile (A)
and water containing 0.1% trifluoroacetic acid (0.1% TFA, B) were employed
as the mobile phase for the gradient elution. The drifttube temperature
and flow rateofcarriergas (N2) were 50℃ and 1.5 L·min-1, respectively.
Under the optimum conditions, good linear regression equations of six
analytes were obtained with the detection limits ranging from 0.2 to 0.5
µg·mL-1. The proposed method has been applied successfully for the
quantification of GSH and PCs in Perilla frutescens (a cadmium
hyperaccumulator) under cadmium stress. The recoveries were between 82.9%
and 115.3%.