GENOME SEQUENCE ANALYSIS OF IN VITRO AND IN VIVO PHENOTYPES OF BUNYAMWERA AND NGARI VIRUS ISOLATES FROM NORTHERN KENYA

The viral isolates including the parental WT, i.e. mixture of SP and LP, were used to infect 90% confluent monolayers of Vero cells at a multiplicity of infection of 0.01. Aliquots of tissue culture fluid were collected at different timepoints and titers determined by plaque assay. The experiment was replicated thrice.The statistical package R was used for fitting exponential growth data using the Kruskal–Wallis test. The detection of correlated error structure in the growth curve data was carried out as follows; the log-transformed data was fit to linear mixed effects models using R, and an AR1 model was determined to fit the data better than a repeated measures model.