Impact of warming on greenhouse gas production and microbial diversity in anoxic peat from a Sphagnum-dominated bog (Grand Rapids, Minnesota, USA)

Here we explore the impact of experimental warming on the microbial community structure associated with peat. The diversity and composition of peat associated bacterial communities composition was determined by applying a high-throughput sequencing-based protocol that targets PCR-generated amplicons from the V4 variable regions of the SSU rRNA gene using the primer set 515F (5'-GTGCCAGCMGCCGCGGTAA) and 806R (5'-GGACTACHVGGGTWTCTAAT)