FgHtf1 regulates the differentiation of phialides and subsequent macroconidiation.

(A) Wild-type strain PH-1 and complementation strain ΔFghtf1-Com produced abundant macroconidia borne on terminal phialides while the ΔFghtf1 mutant produced aberrant terminal phialides but failed to form macroconidia. tp, terminal phialides; ma, macroconidia; cp, conidiophore; h, hyphae. Bar = 20 µm. (B) Fluorescence staining of nuclei with DAPI demonstrated that phialide-like structures in wild type were uninucleate (white arrow). Bar = 20 µm. (C) Fluorescence staining of nuclei with 4′6-diamidino-2-phenylindole (DAPI) observes clumps consisting of hyphal segments in ΔFghtf1 mutant. Bar = 20 µm.