Spore viability of the different rec114 alleles in various genetic backgrounds.

Spore viability was assessed following 2 day incubation on sporulation medium (SPM) plate at 30°C. Generally, 160 spores were scored for each strain except for those with (*) where 320 spores were analyzed. Viability was indicated as the fraction of viable spores over the total dissected. Abbreviations: T; threonine, S; serine, A; alanine, D; aspartic acid, E; glutamic acid, ND; not determined.1Nature of mutations in rec114 alleles analyzed.2Relevant genotypes of the strains to which REC114, rec114-8A, or the four different rec114-phosphomimetic alleles in the “REC114 allele” column were introduced to assess potential genetic interaction(s).3Homozygous diploids expressing the indicated REC114 or rec114 alleles in an otherwise WT background.4Heterozygous diploids expressing a single copy of the indicated REC114 or rec114 alleles; the other allele is rec114Δ.