Thermodynamic parameters for the association of alkaloids with tRNA from ITC at different salt concentrationsa.

aAll the data in this table are derived from ITC experiments conducted in citrate-phosphate buffer of different [Na+], pH 7.0 and are average of four determinations. Ka and ΔH0 values were determined from ITC profiles fitting to Origin 7.0 software as described in the text. The values of ΔG0 and TΔS0 were determined using the equations ΔG0 = −RT lnKa and TΔS0 = ΔH0 -ΔG0. ΔG0pe and ΔG0t are the polyelectrolytic and non polyelectrolytic contribution to ΔG0. All the ITC profiles were fit to a model of single binding.