Poplar transcriptome sequencing

The concentration of exogenous nitrogen and calcium can significantly regulate plant growth and photosynthesis, and mutual affect the absorption and utilization. However, whether there is an optimal nitrogen-calcium ratio (N:Ca ratio) in poplar seedling growth, physiological adaptation, and the transcriptome change mechanism remains unclear. In this study, three different N:Ca ratio were set in poplar seedlings, and physiological and transcriptomic methods were used to study the molecular mechanism of poplar growth under the condition of nitrogen-calcium synergy and to determine the optimal N:Ca ratio of poplar seedling growth. The results of this study showed that the growth condition of poplar seedlings reached a maximum for the treatment with a N:Ca ratio of 2, which was significantly different than the two other treatments (P<0.05). For the lowest N:Ca ratio, poplar seedlings can ensure normal development by regulating photosynthesis, while the highest N:Ca ratio, it can regulate nitrogen metabolism. These results contribute to a better understanding of the molecular mechanisms by which poplar seedlings respond to different ratios of N:Ca. This study provides a valuable basis for exploring the synergistic effects of nitrogen and calcium on the growth of poplar shelterbelts.