Temporal atlas of renal immune cells in crescentic glomerulonephritis

During glomerulonephritis, the phenotype, proliferation, differentiation and function of infiltrated immune cells are affected by the inflammatory context and changed during the course of glomerulonephritis. To comprehensively and temporally understand these characteristics of infiltrated immune cells, we performed single cell RNA sequencing and single cell TCR sequencing from healthy mice and mice with cresentic glomerulonephritis at day 3, day 7 and day 14. Renal CD45+ immune cells and CD3+ T cells were sorted from mouse kidneys with cGN at different timepoints. Sorted cells were loaded for library preparation by using 10x single cell RNA sequencing and 10x single cell TCR sequencing.