Functional unloading leads to metabolic and functional disorders in FAPs in vitro. Functional unloading leads to metabolic and functional disorders in FAPs in vitro

In our study, we used hindlimb unloading rat model to study fibro-adipogenic progenitor cells (FAPs) condition in muscle atrophy. We have purified FAPs from m. soleus from control rats and after 7 and 14 days of immobilization (HS7, HS14). Also, we performed adipogenic differentiation of FAPS in vitro during 3 days. Overall design: Libraries were prepared with TruSeq Stranded mRNA kit, samples were RNA-sequenced on Illumina NextSeq 2000, read length 51 bp.