Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin accessibility and looping [RNA-Seq 2]

During the human cord blood CD34+ cell differentiation, expression of the genes which contribute to erycyte maturation are increased, inculding ALAS2, SLC25A37, GYPA and KLF1. ETO2 functions as a transcription repressor and is required for the erythrocyte maturation and the hemoglobin switch. During mouse embryonic erythropoiesis, RNA-seq data in E8.5 yolk sac /E12.5, E14.5 fetal liver cells indicated that eto2 promoted a critical developmental transition and played an important role in globin switch from embryonic to adult ß-globin transcription since its function is essential for erythorid maturation regulators (Alas2,Slc25a37,Epb42,Gypc,Klf1) and globin genes (Hbb-y and Hba-x) regulation. Overall design: RNA-seq was performed using CD34 cells at differentiation day 8, 10 and 12 to identify the key genes essential for erythrocyte maturation. RNA-seq was also perfromed using embryonic day 8.5 (E8.5) yolk sac and E12.5 fetal liver cells between control and Eto2 knockout mice to identify the differentially expressed genes which are essential for mouse development and embryonic erythropoiesis.