Supporting data for “Site-specific interactome profiling with chemically synthetic phosphopeptides ”

My PhD study primary contains three parts:
Part 1. A new AP-MS technique (FISAP) was developed and it significantly reduced the sample amount and process time for potential large scale applications.

Part 2. Study site-specific phosphotyrosine (pTyr) dependent interactome for the central linear region between the PTB and SH2 domains of the adaptor protein Shc1 with site-specific phosphorylation.

Part 3. Applied FISAP to study the pTyr-dependent interactome of 9 immune co-inhibitory receptors, which are important targets for current tumor immunotherapy.

The quality control of these pulldowns and MS data are included, and the synthesis work and design of the ligation sites of the Shc1 long peptide and 9 immune receptor tails were
organised in the Figure and description word file. The raw files generated from Waters UPLC-MS and LCQ-Fleet of the final product of each long peptide for reference are separately uploaded in zip format together. To read the raw file, software Masslynx 4.1 from Waters or Xcalibur 4.0 from ThermoFisher are needed.

我的博士研究主要包含三个部分：
第一部分：开发了一种新的AP-MS技术（FISAP），显著降低了样本量和处理时间，适用于潜在的规模化应用。
第二部分：针对适配蛋白Shc1中PTB和SH2结构域之间的中心线性区域，研究了特定位点磷酸化依赖的磷酸酪氨酸（pTyr）相互作用组。
第三部分：将FISAP应用于研究9种免疫共抑制受体的pTyr依赖性相互作用组，这些受体是当前肿瘤免疫治疗的重要靶点。
这些下拉实验和质谱数据的质量控制被纳入其中，Shc1长肽和9种免疫受体尾的连接位点合成工作及设计在图和描述性文字文件中组织。每个长肽最终产品的原始文件分别以zip格式上传，以供参考。读取原始文件需要使用Waters UPLC-MS的Masslynx 4.1软件或ThermoFisher的Xcalibur 4.0软件。