Probiotic Limosilactobacillus Fermentum 3872 Raw sequence reads

The aim of this study is to comprehensively analyze the genome of probiotic bacterial strain from commercially available yogurt sample using the next-generation sequencing approach. The strain Limosilactobacillus Fermentum 3872 from commercially available yoghurt sample was cultured on Man Rogosa Sharpe (MRS) agar at 37 C under anaerobic conditions using the Yuejin anaerobic incubator. The pure bacterial colonies after several round of sub-culturing were used for Gram staining, cell morphology, etc. The same colonies from agar plates were grown overnight in MRS broth followed by DNA extraction through Tiangen bacterial DNA isolation kit. Further, Qubit fluorometer was used to assess quantity of extracted DNA. DNA Libraries for high-throughput sequencing were prepared through commercial Vazyme TruePrep DNA Library Prep Kit V2 for Illumina. For sequencing, Illumina HiSeq-2000 sequencing platform was used.