LIN28B is a barrier to the maturation of human embryonic stem cell derived pancreatic ß cells

Comparative RNA and small RNA sequencing analysis of in vitro differentiated ß like cells, in vivo matured ß cells, adult islet cells, and Lin28 deficient in vitro differentiated ß like cells Overall design: We performed RNA-seq and small-RNA seq on human ESCs differentiated into ß like cells in vitro, same cells transplanted into kidney capsule of mouse recipients, and fresh human islets. We also performed RNA-seq on hESCs genomically modified with dox inducible CRISPR knockout of LIN28 and then differentiated into ß like cells plus/mius doxycycline