<b>Phytogenic modulation of cellular heat stress markers: effects of turmeric and ginger extracts in chicken hepatocytes</b>

Heat stress represents a challenge of growing significancy in poultry industry, affecting animal health and productivity. In the present study, effects of turmeric (<i>Curcuma longa</i>) and ginger (<i>Zingiber officinale</i>) extracts on heat shock response in a primary chicken hepatocyte culture model were investigated. Cell cultures were exposed to 43^oC heat stress for 1 hour without supplementation, or in the presence of either turmeric or ginger extract. Cytokine (IL-6, IL-10, IFN-γ, M-CSF and RANTES) concentrations, as well as HSP27, HSP70, and HSP90 levels, were measured after the treatment period. Turmeric treatment significantly increased HSP27 and HSP90 concentrations and reduced HSP70 levels. Moreover, it elevated the concentrations of IL-6, IL-10, IFN-γ, and M-CSF, indicating an enhanced cellular immune status. Ginger-treated groups, on the other hand, showed no significant changes in the markers studied, except for an increase in RANTES levels. Our findings suggest that turmeric supplementation is more effective than ginger in mitigating the cellular effects of heat stress in chicken hepatocytes. These results contribute to a better understanding of heat stress biology in poultry and highlight the need for further studies to fully elucidate the protective mechanisms and practical applications of turmeric and ginger supplementation under thermal challenges.

热应激已成为家禽养殖业中日益凸显的严峻挑战，严重影响畜禽健康与生产性能。本研究旨在探究姜黄（*Curcuma longa*）与生姜（*Zingiber officinale*）提取物对原代鸡肝细胞培养模型热休克反应的影响。将细胞培养体系分为三组：空白对照组（无提取物添加）、姜黄提取物组与生姜提取物组，各组均于43℃环境下接受1小时热应激处理。处理结束后，检测各组细胞的细胞因子（IL-6、IL-10、IFN-γ、M-CSF及RANTES）浓度，以及热休克蛋白HSP27、HSP70与HSP90的表达水平。实验结果表明，姜黄提取物处理组可显著上调HSP27与HSP90的表达水平，并下调HSP70的表达；同时该处理还可上调IL-6、IL-10、IFN-γ及M-CSF的浓度，提示细胞免疫状态得到增强。与之相对，生姜提取物处理组的各项检测指标均无显著变化，仅RANTES水平出现升高。本研究结果显示，在缓解鸡肝细胞热应激的细胞层面效应方面，姜黄提取物的干预效果优于生姜提取物。上述研究结果有助于深化对家禽热应激生物学机制的认知，同时也提示未来需开展更多研究，以全面阐明姜黄与生姜提取物在热应激条件下的保护机制及实际应用价值。

搜集汇总

背景与挑战

背景概述

该数据集研究姜黄和生姜提取物对鸡肝细胞热应激标记物的调节作用。实验通过比较无添加、姜黄和生姜处理下的细胞反应，发现姜黄能显著调节热休克蛋白（如增加HSP27和HSP90、降低HSP70）并提升细胞因子水平，增强细胞免疫状态，而生姜效果有限。结果表明姜黄在缓解热应激细胞效应方面比生姜更有效，为家禽热应激生物学研究和植物源补充剂应用提供了数据支持。