Supplementary Material for: Scoping Review: Integration of the Major Mechanisms Underlying the Regulation of Arteriolar Tone

Cardiovascular diseases (CVD) remain the leading cause of morbidity and mortality worldwide. Arteriolar tone regulation plays a critical role in maintaining appropriate organ blood flow and perfusion distribution, which is vital for vascular and overall health. Impaired arteriolar function has been associated with the evolution and manifestation of various CVD conditions, emphasizing the importance of understanding arteriolar reactivity. This scoping review aims to explore the interplay between five major regulators of arteriolar tone - metabolism (adenosine), adrenergic control (norepinephrine), myogenic activation (intravascular pressure), perivascular oxygen tension, and intraluminal flow rates. Specifically, the aim was to address how arteriolar reactivity changes in the presence of other vasoactive stimuli and by what mechanisms. The review focused on animal studies that investigated the impact of combining two or more of these stimuli on arteriolar diameter. 848 articles were identified through MEDLINE and EMBASE database searches, and 38 studies were included in the final review. The results indicate that arteriolar reactivity is influenced by multiple factors, including competitive processes, structural limitations, and indirect interactions among stimuli. Additionally, the review identified a lack of research involving female animal models and limited insight into the interaction of molecular signaling pathways, which represent gaps in the literature.