Mouse PAb and MAb reactions against DENV-2 virions and NS1 glycoproteins.

aMouse monoclonal antibodies (MAbs) specific for the NS1 (2A5.1), E (2C5.1) or prM (2A4.1) glycoproteins or polyclonal antibodies (PAbs) generated against DENV-2 infections or immuno-affinity purified DENV-2 NS1 glycoproteins, and which were collected immediately prior to challenge with either the DENV-2 NG-C or NSx strains. bImmunogen as either the immuno-affinity purified NS1 glycoprotein (NS1 gp) of the DENV-2 16681, NG-C or NSx strains, ovalbumin (control protein) or repeated live DENV-2 (NG-C strain) infections. cThe mean reciprocal log10 50% end-point ELISA titer (1/log10t50) and standard deviation (s.d.) of pools of mouse PAbs or MAbs, against the immuno-affinity purified NS1 glycoproteins of the DENV-2 NG-C or NSx strains, ovalbumin (control protein) or purified DENV-2 (NSx strain) virions, when detected using peroxidase-labelled goat anti-mouse IgG (H&L) second antibodies. Mouse PAbs which had higher ELISA titers against the DENV-2 virions of the NSx strain than its NS1 glycoprotein are underlined.