Gene expression profile of the major phenotypic subsets of tissue-resident and tissue disease-associated macrophages from healthy and nephritic NZB/W lupus prone mice respectively in comparison to disease-monocyte transcriptional profile

We employed bulk RNA-sequencing to compare gene expression between the two major phenotypic subsets of disease-associated macrophages based on MHCII protein expression as well as tissue-resident macrophages and disease-monocytes. Overall design: Different clusters of kidney macrophages and monocytes were isolated by Fluorescence-activated cell sorting (FACS) and depended and analyzed using bulk-RNAseq.