RNA sequencing of human neural progenitor cells, SK-N-SH, following CHD8 knockdown

Chromodomain helicase DNA-binding protein 8 (CHD8) was previously identified as a leading autism spectrum disorder (ASD) candidate gene by whole-exome sequencing and subsequent targeted-sequencing studies. The present study used small interfering RNA-mediated knockdown of CHD8 in the human neural progenitor cell line, SK-N-SH, in order to determine the consequences of altered expression of this gene. RNA sequencing was performed following CHD8 knockdown to identify differentially expressed genes and affected molecular pathways resulting from this manipulation. This study sheds light on the functional consequences of altered CHD8 expression and may provide insight into the molecular underpinnings of autism spectrum disorder.