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In vitro construct of long artificial DNA sequences

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP145947
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Herein we propose a fully in vitro iterative method based on Golden Gate Aseembly to faithfully construct a long synthetic DNA molecule starting from commercially available double-stranded DNA. The synthetic DNA molecules can be used for any common biological purpose or to store information. We took advantage of long DNA molecule sequencing offered by ONT technology to assess the different assembly steps of our procedure.
创建时间:
2023-03-26
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