Data Antibacterial Activity of Betadine (Jatropha multifida L.) Stem Extract on Pseudomonas aeruginosa Growth In-Vitro

Based on the results of research that has been carried out on the growth of Pseudomonas aeruginosa, it was found that there was a difference in the diameter of the inhibition zone on a petri plate that had been planted with Pseudomonas aeruginosa bacteria between the addition of Betadine stem sap extract, gentamicin and DMSO. The results of the tests carried out using the ANOVA test obtained the value of p = 0.000 < (0.05), which means Ho is rejected. Thus, there was a significant difference in the inhibition zone with the addition of Betadine stem sap extract, gentamicin, and DMSO. Ha accepted means that Betadine (Jatropha multifida L.) stem sap extract has antibacterial activity against the growth of Pseudomonas aeruginosa bacteria in vitro. The method used in this study is the Kirby-Bauer disc diffusion method. The selection of this method was based on the fact that the diffusion method is a method that is fast, easy, simple in its operation and produces effective results to see the antibacterial activity of a material.40 This method produces an inhibition zone. The zone of inhibition is a clear area or region that appears around the paper disc. The larger the diameter of the zone, the greater the antibacterial power. The factors that affect the size of the diameter of the inhibition zone are the culture medium, diffusibility, sensitivity of microorganisms, incubation conditions and drug stability. .43 In this study, the researcher used dimethyl sulfoxide (DMSO) as a solvent. DMSO has the chemical formula (CH3)2SO. DMSO is a natural chemical by-product of papermaking. DMSO is able to dissolve polar and non-polar compounds. DMSO was used as a solvent for pharmacological substances and as a control group for testing natural products. DMSO is also known to have no antibacterial activity so it will not bias the results.41,42 The results of this study indicate that the inhibition zone formed in the negative control (DMSO) which was also used as a solvent was 0 mm. This indicates that the use of DMSO solvent did not affect the antibacterial test results of Pseudomonas aeruginosa.