Temporal transcriptome in Pou5f3, Nanog and Sox19b mutant zebrafish embryos

To understand the role of Pou5f3, Sox19b and Nanog during zebrafish zygotic genome activation, RNA-seq time-series for single, double and triple maternal-zygotic (MZ) zebrafish mutants for Pou5f3, Nanog, Sox19b, as well as the wild-type. Overall design: RNA samples were collected at eight time points: 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6 hours post-fertilization (hpf). For the mutants MZspg (Pou5f3), MZsox19b, MZnanog, MZsox19bspg and MZsox19bnanog and four biological replicates of the wild-type the single-end 50 bp sequencing was performed with the depth 35M reads per sample. For the mutants MZnanogspg, MZtriple (triple mutant for Pou5f3, Sox19b and Nanog) and for six biological replicates of the wild-type paired-end 150 bp sequencing was performed with 58M reads per sample.