Anti-inflammatory metabolites from the mangrove endophytic fungus <i>Diaporthe pseudooculi HHUF</i> 30617

Certain mangrove endophytic fungi have unique physiological mechanisms, such as anti-inflammatory properties. A new polyketone, namely <i>N</i>-acetoxyethyl-3-(3,5-dimethyl-1,3-heptadienyl)-5-chloro-7-methyl-7-acetoxy-6,8-(2<i>H</i>,7<i>H</i>)-isoquinolinedione (<b>1</b>), and 17 known compounds (<b>2</b>–<b>18</b>) were isolated from the mangrove endophytic fungus <i>Diaporthe pseudooculi HHUF</i> 30617. The structure of the new compound was elucidated using nuclear magnetic resonance (NMR) spectroscopy and high-resolution electrospray ionisation mass spectroscopy (HRESI-MS). The anti-inflammatory activities of the compounds were evaluated using the zebrafish migratory fluorescent cell count assay. The results suggest the potential of the above compounds for treating inflammation. These findings aid the development of natural marine products based on mangrove endophytic fungal metabolites to counter conditions such as copper toxicity.

部分红树林内生真菌具备独特的生理机制，例如抗炎特性。研究人员从红树林内生真菌拟眼拟茎点霉<i>Diaporthe pseudooculi</i> HHUF 30617中分离得到1个新聚酮类化合物，即<i>N</i>-乙酰氧基乙基-3-(3,5-二甲基-1,3-庚二烯基)-5-氯-7-甲基-7-乙酰氧基-6,8-(2<i>H</i>,7<i>H</i>)-异喹啉二酮（<b>1</b>），以及17个已知化合物（<b>2</b>–<b>18</b>）。借助核磁共振（nuclear magnetic resonance）波谱法与高分辨率电喷雾电离质谱法（high-resolution electrospray ionisation mass spectroscopy, HRESI-MS），明确了该新化合物的化学结构。研究采用斑马鱼迁移荧光细胞计数法，对上述化合物的抗炎活性进行了评价。实验结果表明，此类化合物具备治疗炎症性疾病的应用潜力。本研究发现可为基于红树林内生真菌代谢产物开发天然海洋药物以对抗铜中毒等病症提供助力。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于从红树林内生真菌Diaporthe pseudooculi HHUF 30617中分离的抗炎代谢物，包括一种新的聚酮类化合物和17种已知化合物，其结构通过核磁共振和质谱技术解析。研究利用斑马鱼模型评估了这些化合物的抗炎活性，结果表明它们具有治疗炎症的潜力，为开发基于真菌代谢物的天然海洋产品提供了基础。