Insect hypovirulence-associated mycovirus confers entomopathogenic fungi with enhanced resistance against phytopathogens

Mycoviruses can alter the biological characteristics of host fungi including decreasing or enhancing virulence or pathogenicity of phytopathogens and entomopathogenic fungi (EPF). As an extensively used EPF, Beauveria bassiana could not only directly control pests, but also improve plant resistance against plant disease through endophytic colonization. However, most studies on the mycoviruses found in B. bassiana have focused on the effects of the viruses on the virulence of host fungi toward insect pests, with relatively few reports on the effects to the host fungi with regard to plant disease resistance in hosts. The present study investigated the effects of the mycovirus Beauveria bassiana chrysovirus 2 (BbCV2) virus infection on host biological characteristics, additionally, we evaluated antagonistic activity of BbCV2 against phytopathogenic fungi (Sclerotinia sclerotiorum and Botrytis cinerea) in vitro and their associated diseases both in in vitro leaves and in pot experiments.  O..., Evaluation of the effects ofBbCV2 virus infection on host biological characteristics
For the growth rate test, 2 μL of B. bassiana suspension at a concentration of 5 × 104 conidia/mL was added to the center of each 20 mL potato dextrose agar (PDA) plate, and cultured in a constant temperature incubator at 26±1℃, with 70±1% relative humidity (RH), and a 16-h light:8-h dark photoperiod. The colony diameter was measured using the cross over method for 12 d, and each strain had five replicates. Colony growth rate was calculated according to the following formula [25]: colony growth rate (mm/d) = (final colony diameter - initial colony diameter)/ culture time, and the size and morphology of the colony were recorded by taking photos.
For the spore production test, 100 μL of B. bassiana suspension liquid at a concentration of 1 × 107 conidia/mL was added to the center of each 20-mL PDA plate. Subsequently, the suspension was blended evenly and placed in a constant temperature incubator at 26±1..., , # Insect hypovirulence-associated mycovirus confers entomopathogenic fungi with enhanced resistance against phytopathogens

[https://doi.org/10.5061/dryad.7sqv9s516](https://doi.org/10.5061/dryad.7sqv9s516)

The present study investigated the effects of the mycovirus *Beauveria bassiana* chrysovirus 2 (BbCV2) virus infection on host biological characteristics, additionally, we evaluated antagonistic activity of BbCV2 against phytopathogenic fungi (*Sclerotinia sclerotiorum* and *Botrytis cinerea*) *in vitro* and their associated diseases both in *in vitro* leaves and in pot experiments.

## Description of the data and file structure

We provided data for Processed and Raw, we also provided Software files for Figure 1-7.

#### Figure\_1\_raw

This file contains  raw datasets for 'Effects of BbCV2 on the biological characteristics of host fungi. (A) Growth rate. (B) Conidiation per unit area. (C) Biomass.'

#### Figure\_1\_prosecced

This file contains  prosecced datasets for 'Effects of ...