Differential midgut transcriptomics comparing nutritional status reveals a crucial role for V-ATPase subunit a and Niemann-pick 1b protein in the digestive physiology of the desert locust.

The main objective of this study was to analyze the general midgut transcript profile of Schiustocerca gregaria as well as changes in midgut gene expression during different stages of feeding. We compared three different time points of the digestive process: no active digestion (24 h after feeding), the initial stages of feeding (10 min after feeding) and active digestion in the midgut (2 h after feeding). Overall design: Samples are RNA extracts of the desert locust midgut. We compared 3 timepoints. Each timepoint consists of 6 biological replicates.