Global gene expression profile of food production grade germinating soybeans

Gene expression of food-production grade germinating Glycine max (L.) Merr. (soybean) was examined using RNA-seq. Soybean varieties from commercial production (GL 3494, OAC Kent) and bioresource (Williams82, Jack) grades were germinated at 27 Celsius or 40 Celsius, 48 h for comparison. RNA-seq analyses on a single individual seed indicated that the gene expression profile is significantly distant apart when germinated at different temperature as compared with among different soybean varieties. More than 16,430 genes with different expression level (FPKM+1 ratio more than 2 and less than 0.5) were identified between germinating temperatures at 27 Celsius and 40 Celsius, whereas approximately one-tenth (more than 1,595 genes) were found among different soybean varieties.